The Raman hyperspectral imaging system can molecularly fingerprint any material or mixture of materials (solids, liquid, or gaseous) that are Raman active. In our lab, the Raman system is hosted by a Newport vibration control table and is equipped with a high accuracy motorized XY stage for chemical mapping (step size of ≥ 0.1 µm), at both the micro and macro scales. The system has confocal imaging capabilities in 2-D and 3-D and can collect both point Raman spectra and Raman maps after excitation with a laser light (~633 nm and 532 nm). Chemometric approaches can then translate the Raman spectral maps into Raman chemical images containing both qualitative and quantitative information about the chemical composition and distribution of the target material(s) within the sample. At 100x magnification, each pixel (0.1-1 µm) of a Raman hyperspectral image can provide a full Raman spectrum (100-4000 cm-1) of that pixel’s spatial area, with high spectral resolution (0.1-1.0 cm-1).